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肝細胞生長因子(HGF)檢測試劑盒

參考價	￥2008.00

參考價

￥2008.00

具體成交價以合同協(xié)議為準

公司名稱杭州昊鑫生物科技股份有限公司
品牌云克隆
型號SEA047Hu
所在地杭州市
廠商性質(zhì)代理商
更新時間2024/7/11 14:45:57
訪問次數(shù)130

產(chǎn)品標簽:

酶聯(lián)免疫吸附試驗法

規(guī)格

48T

2008.00元

10000 盒可售

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杭州昊鑫生物科技股份有限公司成立于2009年初，是一家面向生命科學(xué)領(lǐng)域，從事科研機構(gòu)、高校、院所及生產(chǎn)企業(yè)所需科研試劑、耗材，儀器銷售和服務(wù)面向全國。

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展開顯示更多內(nèi)容

胎牛血清、MCE抑制劑激動劑、RNA提取試劑盒、ELISA試劑盒、重組蛋白

產(chǎn)品簡介在線詢價

產(chǎn)地	進口	規(guī)格	48T
級別	化工級	證書	ISO系列證書

本試劑盒運用雙抗體夾心 ELISA 法定量測定人血清、血漿、組織勻漿、細胞裂解液、細胞培養(yǎng)上清或其它相關(guān)生物液體中 HGF 含量

肝細胞生長因子(HGF)檢測試劑盒產(chǎn)品信息

肝細胞生長因子(HGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

編號SEA047Hu
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
實驗方法雙抗夾心
反應(yīng)時長3h
檢測范圍31.2-2,000pg/mL
靈敏度最小可檢測劑量小于等于11.5pg/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格48T96T 96T*5 96T*10 96T*100

特異性

本試劑盒用于檢測肝細胞生長因子(HGF)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的肝細胞生長因子(HGF)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	87-94	91
EDTA plasma(n=5)	94-101	97
heparin plasma(n=5)	93-102	97

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的肝細胞生長因子(HGF)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中肝細胞生長因子(HGF)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	91-101%	80-94%	85-103%	96-103%
EDTA plasma(n=5)	78-97%	94-103%	80-90%	83-94%
heparin plasma(n=5)	90-103%	96-103%	87-103%	91-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實驗原理

將肝細胞生長因子(HGF)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的肝細胞生長因子(HGF)與連接于固相載體上的抗體結(jié)合，然后加入生物su化的肝細胞生長因子(HGF)抗體，將未結(jié)合的生物su化抗體洗凈后，加入HRP標記的親和素，再次洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的肝細胞生長因子(HGF)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。

參考文獻

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