生物發(fā)酵罐Biological Fermentor
發(fā)酵罐概述Overview Of Fermentation Tank
常見的發(fā)酵罐分為固體發(fā)酵罐和液體發(fā)酵罐。發(fā)酵罐根據(jù)容積和用途不同,分為種子罐(菌種發(fā)酵罐)和發(fā)酵罐。發(fā)酵罐根據(jù)需氧類型的不同又分為通風式發(fā)酵罐和厭氧發(fā)酵罐。發(fā)酵罐、種子罐,有碳鋼制、不銹鋼、高鎳基合金等,主要用于生化發(fā)酵生產(chǎn)。如生產(chǎn)味精、檸檬酸、酶制劑、溶劑、制藥、食品等行業(yè)。
一、發(fā)酵罐的基本條件Basic Conditions Of Fermentation Tank
機械攪拌發(fā)酵罐是發(fā)酵工廠常用類型之一。它是利用機械攪拌器的作用,使空氣和醪液充分混合,促使氧在醪液中溶解,以保證供給微生物生長繁殖、發(fā)酵所需要的氧氣。使用發(fā)酵罐的基本要求:
1、發(fā)酵罐應具有適宜的徑高比。發(fā)酵罐的高度與直徑之比一般為1.7~4倍左右,罐身越長,氧的利用率較高。
2、發(fā)酵罐能承受一定壓力。由于發(fā)酵罐在消毒及正常工作時,罐內(nèi)有一定的壓力(氣壓與液壓)和溫度,因此罐體各部件要有一定的強度,能承受一定的壓力。
3、發(fā)酵罐的攪拌通風裝置使氣液充分混合,保證發(fā)酵液必須的溶解氧。
4、發(fā)酵罐應具有足夠的冷卻面積。微生物生長代謝過程放出大量的熱量,為了控制發(fā)酵過程不同階段所需的溫度,應裝有足夠的冷卻面積。
5、發(fā)酵罐內(nèi)應盡量減少死角,避免藏垢積污,易滅菌,避免染菌。
6、攪拌器的軸封應嚴密,盡量減少泄漏。
二、發(fā)酵罐罐體尺寸比例Size Proportion Of Fermentation Tank
罐體各部分的尺寸有一定的比例,罐的高度與直徑之比一般為1.7~4倍左右。
發(fā)酵罐通常裝有兩組攪拌器,兩組攪拌器的間距S約為攪拌器直徑的三倍。
對于大型發(fā)酵罐以及液體深度HL較高的,可安裝三組或三組以上的攪拌器。
最下面一組攪拌器通常與風管出口較接近為好,與罐底的距離C一般等于攪拌器直徑Di,但也不宜小于0.8Di,否則會影響液體的循環(huán)。
三、發(fā)酵罐的結(jié)構(gòu)Structure Of Fermentation Tank
好氣性機械攪拌發(fā)酵罐是密封式受壓設(shè)備,主要部件包括罐身、攪拌器、軸封、打泡器、聯(lián)軸器、中間軸承、空氣吹泡管(或空氣噴射器)、擋板、冷卻裝置、人孔以及管路等。
系統(tǒng)由發(fā)酵罐,空氣處理系統(tǒng)、蒸汽凈化系統(tǒng)、電器控制系統(tǒng)、恒溫系統(tǒng)及管路、閥門、輔助系統(tǒng),傳感器與一次儀表系統(tǒng),下位機控制系統(tǒng)(現(xiàn)場PLC控制系統(tǒng),二次儀表),上位機系統(tǒng)以及臺架等組成。
四、液體發(fā)酵罐技術(shù)參數(shù)/Technical Parameter
公稱體積 Nominal Volume | 5~3000L |
裝液系數(shù)Liquid Loading Coefficient | 65-80%, Optimum 70% |
罐體配置 Can Body Configuration | 不銹鋼SUS316L/SUS304材質(zhì); Stainless steel SUS316L / SUS304 Material; Jacket temperature control and optimization of diversion design; Internal surface polishing, RA ≤ 0.6 μ m; External surface polishing or matte treatment (glass shot blasting); Long strip mirror with large viewing angle, complete interfaces of pH electrode, do electrode and temperature electrode; The tank cover is equipped with flame inoculation port and feeding standby interface, etc |
攪拌系統(tǒng) Mixing System | 頂入式機械攪拌系統(tǒng);無菌機封系統(tǒng);直流攪拌電機(10L及以下)或交流電機; Jacking mechanical mixing system; Aseptic mechanical sealing system; DC mixing motor (10L and below) or AC motor; Standard configuration: 2-layer flat impeller and one-stage high-efficiency defoaming paddle; There are also inclined blades, curved blades, axial flow blades, etc; Adjustable speed, digital setting speed control |
結(jié)構(gòu)方式 Structure Mode | 落地式框架結(jié)構(gòu),節(jié)省空間,萬向腳輪,操作方便 Floor type frame structure, space saving, universal casters, convenient operation |
通氣系統(tǒng)Ventilation System | 深層通氣,全不銹鋼管路; Deep ventilation, all stainless steel pipeline; Glass rotor flowmeter display, pressure display, manual valve adjustment flow; High efficiency sterilizing filter equipped with stainless steel housing, with a filtration accuracy of 0.01 μ m; External compressed air is required, equipped with high-quality water removal and pressure stabilizing devices, to continuously and stably supply gas; Optional mass flow controller for automatic control of air flow |
罐壓控制 Tank Pressure Control | 頂部排氣口配置指針式壓力表顯示罐壓,不銹鋼閥門手動調(diào)節(jié); The top exhaust port is equipped with a pointer pressure gauge to display the tank pressure, and the stainless steel valve is manually adjusted; It can be equipped with inlet pressure transmitter and automatic regulating valve to realize automatic control of tank pressure |
溫度控制/Temperature Control | 在線檢測,數(shù)字化設(shè)定,自動/手動控制自由切換; Online detection, digital setting, automatic / manual control, free switching; German PT-100 temperature probe and digital temperature instrument; Automatic temperature control of electric heating thermostatic water tank and external cooling water |
Ph控制 Ph Control | 在線檢測,1路或2路蠕動泵自動流加堿或酸液進行調(diào)節(jié); Online detection, 1 or 2 peristaltic pumps automatically add alkali or acid for regulation; Imported Mettler (Hamilton) pH electrode, which can be sterilized by high temperature steam |
溶氧(Do) Dissolved Oxygen (Do) | 在線檢測,范圍0-100%或0-200%; Online detection, range 0-100% or 0-200%; Imported Mettler (Hamilton) do electrode, which can be sterilized by high temperature steam; Optional linkage control with speed and ventilation (to be specified when ordering) |
補料控制Replenishment Control | 標配1路/2路等多路蠕動泵時間比例流加補料,流加量累積顯示、記錄 It is equipped with 1-way / 2-way and other multi-channel peristaltic pumps as standard, with time proportional flow feeding and feeding, and the cumulative flow feeding amount is displayed and recorded |
消泡方式Defoaming Mode | 傳導式泡沫電極,泡沫異常狀況自動報警; The conductive foam electrode automatically alarm the abnormal condition of foam. One peristaltic pump is equipped as standard for automatic / manual flow of defoamer, and the cumulative flow dosage is displayed and recorded |
控制系統(tǒng) Control System | 應用工業(yè)級MC-bio控制系統(tǒng),采用西門子PLC控制器; Apply industrial MC bio control system and Siemens PLC controller; 32-bit true color touch screen operation interface with complete functions and stable operation; Another type a simple instrument control system is optional |
滅菌方式/Sterilization Method | 在位滅菌,手動控制,需接外源飽和蒸汽 In place sterilization, manual control, need to connect external saturated steam |
可選配項/Optional Configuration | 1.多路補料 Multiple feeding |
設(shè)備特點Equipment Characteristics | 在位滅菌,安全可靠,培養(yǎng)基濃度準確; In situ sterilization, safe and reliable, accurate medium concentration; No risk of transmission leakage; Large viewing angle longitudinal mirror observation is clear; Various and reliable inoculation methods (standard flame inoculation port, differential pressure inoculation port and other forms are optional); Floor type tank, beautiful and generous; Easy operation and disassembly; Special specifications can be customized. |