DSP-d8,DTSP-d8,Deuterated Crosslinker DSP-d8,氘代交聯(lián)劑

DSP-d8,DTSP-d8,Deuterated Crosslinker DSP-d8,氘代交聯(lián)劑

DSP-d8 deuterated protein crosslinker, Dithiobis(succinimidyl 2,2,3,3,6,6,7,7-propionate-d8), is a cell membrane permeable, homobifunctional, protein crosslinker containing 8 deuterium atoms to provide an 8 Dalton shift by mass spectrometry pared to non-dueterated DSP (DSP-d₀). DSP-d8 deuterated crosslinker has amine-reactive NHS esters at both ends of a cleavable, 8-atom (12.0 angstrom) spacer arm. DSP-d8 deuterated crosslinker contains a reducible disulfide bond (SS-bond) in the spacer arm that makes it a good choice for numerous applications where reversible crosslinking is desired. 

Use the heavy DSP-d8 deuterated protein crosslinking reagent in bination with the light, non-deuterated analog (DSP-d₀) for mass spec studies. 

DSP-d8 deuterated crosslinker must be dissolved in an organic solvent, such as DMSO or DMF, and then may be added to an aueous crosslinking reaction. Because DSP-d8 crosslinker is cell membrane permeable it can be used to crosslink intracellular proteins. 

Molecular Weight: 412.46 
Spacer Length: 12.0 Å 

Alternative names: d8-DSP; DSP-d₈; D8-DSP; Dithiobis(succinimidyl propionate-d8); DTSP-d8 

References:
Tan, L., Turecek, F., Francisco, J. S., & Xia, Y. (2014). Probing the Radical and Base Dual Properties of Peptide Sulfinyl Radicals via Mass Spectrometry. The Journal of Physical Chemistry A, 118(50), 11828-11835. 

Rotunno, M. S., Auclair, J. R., Maniatis, S., Shaffer, S. A., Agar, J., & Bosco, D. A. (2014). Identification of a misfolded region in superoxide dismutase 1 that is exposed in amyotrophic lateral sclerosis. Journal of Biological Chemistry, 289(41), 28527-28538. 

structure：

DSP-d8,DTSP-d8,Deuterated Crosslinker DSP-d8,氘代交聯(lián)劑

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Sulfo-NHS-Acetate,CAS:152305-87-8

Sulfo-NHS-Biotin,CAS:119616-38-5

NHS-Biotin,CAS:35013-72-0

Sulfo-NHS-LC-Biotin,CAS:127062-22-0

NHS-LC-Biotin,CAS:72040-63-2

Sulfo-NHS-SS-Biotin,CAS:325143-98-4

NHS-SS-Biotin,CAS:142439-92-7

Biotin Hydrazide,CAS:66640--6

Biotin-HPDP,CAS:129179-83-5

Iodoacetyl-LC-Biotin,CAS:93285-75-7

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