5-TAMRA Azide|5-TAMRA N3|1006592-61-5

5-TAMRA Azide|5-TAMRA N3|1006592-61-5
TAMRA 熒光染料是在用的多種不同共軛物中Z受歡迎的熒光試劑之一，可標(biāo)記蛋白質(zhì)和寡核苷酸。TAMRA具有吸光的性質(zhì)，它的光譜與幾類常用的熒光化合物重疊，TMR是明亮的橙色熒光基團(tuán)。

5-TAMRA Azide|5-TAMRA N3|1006592-61-5

 

5-TAMRA Azide (also known as TAMRA azide, isomer 5) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.

Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.

5-TAMRA azide is exact replacement of Invitrogen&rsuo;s (Thermo Fisher Scientific) Tetramethylrhodamine (TAMRA) Azide (Tetramethylrhodamine 5-Carboxamido-(6-Azidohexanyl)), 5-isomer

 

               

Abs/Em Maxima	553/575
Extinction coefficient	92,000
Flow cytometry laser line	532 or 555 nm
Microscopy laser line	532 or 555 nm
Spectrally similar dyes	Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight	555.67
CAS	1006592-61-5
Solubility	Water, DMSO, DMF, MeOH
Purity	>98% (HPLC)
Appearance	Red solid
Storage conditions	-20C
Shipping conditions	Ambient temperature

5-TAMRA Azide|5-TAMRA N3|1006592-61-5

 

其他羅丹明熒光染料試劑：

5(6)-Carboxy tetramethyl rhodamine;5(6)-TAMRA                     150347-56-1

5-Carboxytetramethylrhodamine; 5-TAMRA                                     91809-66-4

6-Carboxytetramethylrhodamine;6-TAMRA              91809-67-5

&uot;5(6)-Carboxytetramethylrhodamine succinimidyl ester;5(6)-TAMRA, SE&uot;  246256-50-8

&uot;5-Carboxytetramethylrhodamine succinimidyl ester;5-TAMRA, SE&uot;              150810-68-7

&uot;6-Carboxytetramethylrhodamine succinimidyl ester;6-TAMRA, SE&uot;              150810-69-8

&uot;1,4-dichloro 5-Carboxytetramethylrhodamine&uot;                               198546-47-3

&uot;1,4-dichloro 6-Carboxytetramethylrhodamine&uot;                               407581-83-3

Sulforhodamine 101; Sulforhodamine 640                                   60311-02-6

5-Carboxy-X- rhodamine; 5-ROX                                         216699-35-3

6-Carboxy-X- rhodamine; 6-ROX                                         194785-18-7

5(6)-Carboxy-X- rhodamine; 5(6)-ROX                                          198978-94-8

6 d ROX                            

5 d ROX                            

Rhodamine 101;Rhodamine 640                                            64339-18-0

 

5(6)-TAMRA C6 maleimide 

5-TAMRA C6 maleimide      

6-TAMRA C6 maleimide                    

5(6)-TAMRA Maleimide                           

5-TAMRA Maleimide                        

6-TAMRA Maleimide                               

5-TAMRA alkyne                              

6-TAMRA alkyne                       

5-TAMRA azide                                

6-TAMRA azide                                

5(6)-TAMRA cadaverine                                  

5-TAMRA cadaverine                               

6-TAMRA cadaverine                               

5(6)-TAMRA ethylenediamine                          

5-TAMRA ethylenediamine                       

6-TAMRA ethylenediamine                              

 

 

 

 

 

5-TAMRA TEA Salt     (5-羧基四甲基羅丹明三乙胺鹽)          150322-05-7                

5-TAMRA-NHS        (5-羧基四甲基羅丹明琥珀酰亞胺酯)      3212-17-3                

6-TAMRA TEA salt     (6-羧基四甲基羅丹明三乙胺鹽)           150322-06-8                

6-Aminotetramethylrhodamine     (6-氨基四甲基羅丹明)            80724-18-1                  

5-Aminotetramethylrhodamine    (5-氨基四甲基羅丹明)              80724-17-0,167095-10-5     

6-FAM-C6-OH    6-(N-6-羥基己基)酰胺熒光素                467456-19-5                

5(6)-FAM-C6-OH   5/6-(N-6-羥基己基)酰胺熒光素      9889-37-2；467456-19-5        

Fmoc-Lys(5-FAM)-OH    FAM標(biāo)記的Lys賴氨酸      1242933-88-5              

Fmoc-Lys(5/6-FAM)-OH  5/6-FAM標(biāo)記的Lys賴氨酸    CAS: 1242933-88-5；1266666-04-9)

5(6)-TET    5/6-羧基四氯熒光素   155911-14-1                

5(6)-HEX   5/6-羧基六氯熒光素      155911-16-3                

4-Aminomethyl-6-FAM (6-CAM ) 4-氨甲基-6-羧基熒光素      326802-08-8                

4-Aminomethyl-5-FAM (5-CAM ) 4-氨甲基-5-羧基熒光素      198546-45-1                

Fura-2 ethyl ester   96331-97-4                  

BAPTA tetraethyl ester          73630-07-6                  

Calcein    207124-64-9              

Boc-Statine  58521-49-6                   

Fmoc-Statine        158257-40-0                

Diacetate-5-FAM-NHS    5-羧基二乙酰化熒光素琥珀酰亞胺酯   150206-05-6                

Diacetate-6-FAM-NHS    6-羧基二乙?；療晒馑冂牾啺孵?nbsp;         150206-15-8         

6-Carboxy-X-Rhodamine，SE (NHS) 6-羧基-X-羅丹明琥珀酰亞胺酯   216699-36-4       

5-Carboxy-X-Rhodamine，SE (NHS) 5-羧基-X-羅丹明琥珀酰亞胺酯   209734-74-7  

Dipiv-5-FAM-Osu (NHS)  5-羧基-二特戊酰氧基熒光素琥珀酰亞胺酯              

Dipiv-5-FAM  6-羧基熒光素二特戊酸酯                      

Dipiv-5/6-FAM-C6-OH  5/6-N-(6-羥基己基)酰胺-熒光素二特戊酸酯                       

ATTO 565 meta-isomer(1+)                              

ATTO 565 para-isomer(1+)                              

Fmoc-Glu(EDANS)-OH              193475-66-0