Desthiobiotin-PEG4-Alkyne,脫硫生物素PEG炔烴的作用

Desthiobiotin-PEG4-Alkyne,脫硫生物素PEG炔烴的作用

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly eual specificity but less affinity than biotin (K_d = 10¹¹ vs. 10¹⁵M, respectively). Conseuently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on petitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein plexes with free biotin.

結(jié) 構(gòu) 式：

Desthiobiotin-PEG4-Alkyne,脫硫生物素PEG炔烴的作用

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生物素衍生物目錄

Desthiobiotin-PEG3-Azide

Desthiobiotin NHS Ester

Desthiobiotin-PEG4-Alkyne

TAMRA-Azide-Desthiobiotin

DBCO-PEG4-Desthiobiotin

Biotin-PEG12-NHS Ester

Biotin-Sulfo-NHS Ester

Biotin-NHS Ester

Dde Biotin-PEG4-NHS ester

PC Biotin-NHS ester

Biotin-PEG4-NHS Ester

Biotin-NHS Ester

Sulfhydryl Reactive

UV-Tracer Biotin-Maleimide

Biotin-PEG3-Maleimide

Cleavable Biotin Probes

Dde Biotin-Azide

Dde Biotin Picolyl Azide

Diazo Biotin-Azide

Disulfide Biotin Azide

PC Biotin-Azide

Diol Biotin-Azide

PC Biotin-Alkyne

Dde Biotin-Alkyne

Diol Biotin-Alkyne

Dde Biotin-DBCO

Diazo Biotin-Alkyne

PC DBCO-Biotin

WS PC DBCO-Biotin

Diazo Biotin-DBCO

Disulfide DBCO Biotin

Click Chemistry

Biotin-PEG3-Azide

Biotin Picolyl Azide

TAMRA-Biotin-Azide

Biotin-PEG4-Methyltetrazine

Fluorescein-Biotin-Azide

Cy5-Biotin-Azide

Biotin-PEG4-Alkyne

DBCO-PEG4-Biotin

WS DBCO-Biotin

Biotin-PEG3-Amine

Cy5 Biotin